Mouse Prostate Specific Antigen (PSA) ELISA Kit

Cat.#:

JL10708

Regulatory Status:

RUO

Alternative:

KLK3; APS; KLK2A1; HK3; KLKB1; Seminin; Prostate Specific Antigen; Kallikrein-Related Peptidase 3; Semenogelase; g-Seminoprotein; P-30 Antigen; Plasma kallikrein

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

15.6-1000pg/mL

Sensitivity:

7.6pg/mL

Specificity:

This kit recognizes Mouse PSA in samples. No significant cross-reactivity or interference between Mouse PSA and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates and other biological fluids

Assay Procedure:

Research Area:

Cancer

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| PSA

Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), P-30 antigen, is a glycoprotein enzyme encoded by the KLK3 gene. PSA is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland in men and the paraurethral glands in women.PSA is produced for the ejaculate, where it liquefies semen in the seminal coagulum and allows sperm to swim freely. It is also believed to be instrumental in dissolving cervical mucus, allowing the entry of sperm into the uterus.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse PSA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	95
Plasma(n=8)	92-106	103
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Mouse PSA and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-95	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-102	87-108	105-114
1 : 4	Average (%)	94	98	107